WT PBRM1- TRIPZ-neo

LOCUS       WT PBRM1- TRIPZ-neo               14924 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Mammalian expression, inducible PBRM1.
ACCESSION   .
VERSION     .
KEYWORDS    WT PBRM1- TRIPZ-neo
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14924)
  AUTHORS   Gao W, Li W, Xiao T, Liu XS, Kaelin WG Jr
  TITLE     Inactivation of the PBRM1 tumor suppressor gene amplifies the 
            HIF-response in VHL-/- clear cell renal carcinoma.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Jan 31;114(5):1027-1032. doi: 
            10.1073/pnas.1619726114. Epub 2017 Jan 12.
  PUBMED    28082722
REFERENCE   2  (bases 1 to 14924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..14924
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(1013..1078)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(6005..6031)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(6032..6055)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(6099..6413)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(6102..6126)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    6173..6191
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6209..6227
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6244..6262
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6280..6298
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6316..6334
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6351..6369
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6387..6405
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    6487..6604
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6902..6946)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7131..7364
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7861..7986
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8033..8213
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8231..8429)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8256..8276)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8431..8734
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(8920..9508)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9000..9019)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    9837..9884
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     9909..9928
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(10116..10910)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(10945..11302)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10959..11094
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11259..11279
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(11304..11408)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11435..11793
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11522..11541)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11732..11753
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11816..11835
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11816..11834
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      11855..11990
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    12030..12151
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12041..12060)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     12095..12114
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             12223..12456
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(12528..13322)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    13329..13902
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(13489..13508)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     13718..13735
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     protein_bind    complement(13922..13955)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACAT). loxP511 sites are compatible with each
                     other, but incompatible with wild-type loxP sites."
     CDS             complement(13956..14663)
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     protein_bind    complement(14670..14703)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACAT). loxP511 sites are compatible with each
                     other, but incompatible with wild-type loxP sites."
     promoter        complement(14716..1003)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(14860..14879)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 gcctcactta tccctcccct caccagaggt ccggcgcctg tcgattcagg agagcctacc
       61 ctaggcccga accctgcgtc ctgcgacgga gaaaagccta ccgcacacct accggcaggt
      121 ggccccaccc tgcattataa gccaacagaa cgggtgacgt cacgacacga cgagggcgcg
      181 cgctcccaaa ggtacgggtg cactgcccaa cggcaccgcc ataactgccg cccccgcaac
      241 agacgacaaa ccgagttctc cagtcagtga caaacttcac gtcagggtcc ccagatggtg
      301 ccccagccca tctcacccga ataagagctt tcccgcatta gcgaaggcct caagaccttg
      361 ggttcttgcc gcccaccatg ccccccacct tgtttcaacg acctcacagc ccgcctcaca
      421 agcgtcttcc attcaagact cgggaacagc cgccattttg ctgcgctccc cccaaccccc
      481 agttcagggc aaccttgctc gcggacccag actacagccc ttggcggtct ctccacacgc
      541 ttccgtccca ccgagcggcc cggcggccac gaaagccccg gccagcccag cagcccgcta
      601 ctcaccaagt gacgatcaca gcgatccaca aacaagaacc gcgacccaaa tcccggctgc
      661 gacggaacta gctgtgccac acccggcgcg tccttatata atcatcggcg ttcaccgccc
      721 cacggagatc cctccgcaga atcgccgaga agggactact tttcctcgcc tgttccgctc
      781 tctggaaaga aaaccagtgc cctagagtca cccaagtccc gtcctaaaat gtccttctgc
      841 tgatactggg gttctaaggc cgagtcttat gagcagcggg ccgctgtcct gagcgtccgg
      901 gcggaaggat caggacgctc gctgcgccct tcgtctgacg tggcagcgct cgccgtgagg
      961 aggggggcgc ccgcgggagg cgccaaaacc cggcgcggag gccacgcgtc tacttgtcat
     1021 cgtcatcctt gtaatcgatg tcatgatctt tataatcacc gtcatggtct ttgtagtcac
     1081 ctccctcgag ggccggcctt aattaaacat tttctaggtt gtatgcttgg cgaatgttga
     1141 gggtgtcccg gagcatcaaa tctcgaaggc gccagagggc atctgccatg gtggtgtggg
     1201 cccctttgct tttcagccag tgagagggta ggcggctctc ctgttctttc gacaaatgga
     1261 cgtcgcgtct tcgagctgcc agtgtctgat cccacttgct aatgctgttg gactccgcac
     1321 tgagtccttc aatgtatttc aggtaggcct ctgagtgaag aagccgctgg gtctttggtg
     1381 ggggagctac aaacatgggt gttgttggct gctgtatgac agggggtcca gctggatgtg
     1441 ggccgggata tggaggtggt gcctgctgcc ctggaggccc caaaactccc acctgttgtc
     1501 catatggact tccacccggt gctggagtcc ctaccatagg ggccactcct tggttcatca
     1561 cacccggtgg tgggatgccc gggaggccag gcacacctgg cggaagatgg tggggtggag
     1621 gccccccagg gtgaagtggc tgcatgctgc ccatgctaac aaggccatca actgggccct
     1681 gcaaaggtgg aaggcctggc ggatagccac ccatcatgcc ttcatattct gctttcttgg
     1741 ctgtctcaag atttctccat tctgtcccca ccaggcggct gagctccccg aaagagtagt
     1801 ctgggtgttg ggccttaatc acagccctca tctcactgct gaacaggatg tagccactca
     1861 tgttgatttt ccgtttggag ccttccttct ttgcactgcc tttggcagac tttggggtag
     1921 actgtggggg tgtgtagggc atgaggtcca gctcactggc caggggggtc tgaagctgag
     1981 gtagagaagg aggttcaatg acctcactat cttcttctcc catctcttca atatcatcat
     2041 ctccaccttc taactcggca aatttagctt ctagcaactg gatcttcttt tccagcaaag
     2101 gtgatggctc cttctgagga acaattggtt ttctgaagta gtaaatttca tcatctacca
     2161 ctttagcaga gagtgaaaac ctcttcaatc ctttgaattt cttcatctgc ttgtcgctct
     2221 cattgtagcg gctctcacaa agcagaatgt cattttctgg tatttcagtt ggcctgcagg
     2281 agaggaagtc cttgaatgac aacacagcac actttccgag aatacatgtc atggggcagg
     2341 tttcttccag attactcaga aatacttctt ttttgtagaa catttttgtg ggctcatgct
     2401 ctgtttcttc agggtgaatg aagatggggc cataaaaata tgcagctcca tctcgaaccc
     2461 atactttttc aattctgccc acacgaggac gcaccaggcc atgggacttg atgaagacac
     2521 agtcgccaac cttcagccac atgtcattgt aatggagctg ctcaaagtag tggcaacctg
     2581 gttcaccatt ggacatttcc acagggacat cttctttttc cttttccaag ttaaaattgt
     2641 cttcagctcg actgtcctct gagttgtctg tattcttctc atcatcacct ttatctgcat
     2701 ttgcaaatac agaggccacg cgaaccacag gcagaggcac atcccgaggg acaaacctga
     2761 ctgagctgat gggcatggtc cacagtttaa ttttcttaaa agatttggtt ttggcagaat
     2821 accgtgattc acagacaaaa acatcctcat ctcggaagtt ttctgggcat aacttaaagt
     2881 attccttgac aaacatgacc acacacttgc ctagaatttt actaactgga actttgttgt
     2941 aatagtcact cttaaaaact tctttttcta gaaattttcg tgtagccagg tggaatgttt
     3001 catttggtcg gtaaaaccaa cagccataca accatttttc accagctgaa tcctcccaca
     3061 gtctttcaat acagacgata tgtggttgta ggttggcctc tgcaggttcc acatagacgt
     3121 aatctccaac atggtacatg ctgtttttaa agctacagtc ctggctgtat gtgcgatgta
     3181 agcctgagag gcctgcagca ccagaggaat cttcactctt ttcagcttct cttttttctt
     3241 cttctcgttt tagtttatct tcctctattt cttttggcaa tttttccttt ctctctttct
     3301 ccacatcatt atgcaaatgt tttgtggtat agctgagtgc cggtgaaaga agaatctctc
     3361 catttttgca gagttcatca cgaattttaa taaaaaactg ctgaagttct actgcatctt
     3421 catatatttc tgaatctgtc cgattcatcc ttcttgctcg ttccaatact tcaaacatat
     3481 gctcttgaaa taaatcaagc cgacggtagc gattattttc aacattcttc ctaattatgt
     3541 caaatgtaag gggtggtttg ttaggaaagt tgggatccac agcaggaatt tctgctaaag
     3601 aatcgctgta gcatcttccc tcatcatcct gatgactcat gactgacaca aaaagattgt
     3661 ggataagctc ttgaatcagc aaagtcacat ttgggacatg agagtcctca tctccctcca
     3721 ggtctctgcg tgtttcaagc aggactttgt gtagaacaag agcatctttg tagatcaaag
     3781 actccggctc attgtacgta caggcattat taaacatcat gacaaagtcc tcaaccatag
     3841 agtcaatatc ttggtacttg ttggccatca tgtgacttcg aattttttcc atgtccatgg
     3901 gctttttaat agtcagatag tagtcaggca actcagatct agagggaagc ctcagaaata
     3961 tggcactgag gcggcgaccc ctcttatcag tatagttctt tacagcttca tagacctcat
     4021 ttagtttctg ctgcattgga gtcatgtatt ttgatttttt aggagaaatg ccactcttcc
     4081 tactcagctt gagtttggga gaagccatgt catcatcatc aggcagtggg cccagctctt
     4141 tccttttctc cttgagtaac ttctccagga tatgtgcatc attataaacc tgggagccct
     4201 cctcattata gtgcctggca ttccggaaca tcagcttcat gtcttctatc attccctctt
     4261 caccagcata tttgtcattg cggatgttat gctcaattat tttcaagtcc attggctcca
     4321 agatgatttt ataataatca ggatagtcct ttttggatgg tttaaccata aataggtcac
     4381 aaagtcttct gcctgaacct ggctctcgag cttcaagaac aacattgaat aagattttca
     4441 ttcgctgctt tcttatgttc tttttacttt ttcttttggc actaccagta tcagaggtgg
     4501 ctgaagagat catgctgtct ccgtcctcga tatcgtctct cctggcaagc tctttcttct
     4561 ttgcctgcat aacttgctgc aattttagaa ctcgcttgta gatggctgaa ttgggcacat
     4621 tatagcgttt ggcattttca aacattaaat tcagatcaca ctccaaatga tctaaagttt
     4681 catattcttg attcttcagt tttgttcgga tctgttgtag tgatatgggc attttaattt
     4741 gctggtaata atcagggtat tttttctttg aaggcaaatg gtaaaaaggt tcagctatta
     4801 gctgcccttg gttattccga caactcctaa ctgtgtcata aagctgataa aaaggatttg
     4861 aaacatccat aaaggaagtg atgctttctg cttctgactc tccctcttca tagcgtgcag
     4921 cagctaaagc agcatcttct tcactttctg agccatattg aagtgccatt gtaattgctg
     4981 ataaacgacc tccttgtact gctcttttat tacggtaata cttgctagtg ggatttctct
     5041 cttcaccaag gctgccttta ctgtgtgaag gacctgtcag tctggctgca gccaagttgg
     5101 atggagtcct cattcgaaga cttgacttag ccatttcatg atgttcaatt tcagcctttt
     5161 tcatataaaa tattttttta attgaatttg catccttgaa tacttgagag ccaggctcat
     5221 tataagtttt ggcatttttt gcgaggagat ctatatcttt ggccattgca tgaatacttt
     5281 tgtagcttcc attctgtatc ctctgggcaa tggtcttgag atctataggc tccttaatta
     5341 ttgcataata atctggatat tgcactttag aaggcagttt ctgaaaaagt tcgctaatga
     5401 gacgtcctga tggatttgta gctacaacta tggcttcaag aagctgctcc aggatctcct
     5461 tcaagtaagc tggagaagat ccttcagtca ctgtgccctg attgtcttgc ccatcttcat
     5521 catcatcttc gtcatctgct tctcctttct gaacaaactc atttcttgtt cgaaggtaca
     5581 aatcccagag tttgcaagcg gctttatatt caggagaatc tggcttataa taggactttg
     5641 cattgttaaa aagaagctgg aagtcagcag tcagcaaatt aacatcatca tactcttcca
     5701 tttttagttt ctgttggatt ttcatcaagt caatgggctg agaaaccact tcataatagt
     5761 ctggttgatt tcttcgcttt ggtgccctaa tgaagagctc acagagaagt ctgccctgtt
     5821 catccttata gtctcggatg gtattataga gttcatggca cacggcaata ggatctacag
     5881 ttggaagatt ggaaagtctc ctccttttcc tgcttgggcc tggtgttgac acagaatggt
     5941 gcccatcatc aaagtccccg ctgacactgc tggaagggga ggtagctctt cttctcttgg
     6001 aaccagcgta atctggaaca tcgtatgggt acttgtcgtc atcgtctttg tagtccatga
     6061 attcgtcgac aattcgatcc ggcagtctag attctccagg cgatctgacg gttcactaaa
     6121 cgagctctgc ttatataggc ctcccaccgt acacgcctac ctcgacatac gttctctatc
     6181 actgataggg agtaaactcg acatacgttc tctatcactg atagggataa actcgacata
     6241 cgttctctat cactgatagg gagtaaactc gacatacgtt ctctatcact gatagggagt
     6301 aaactcgaca tacgttctct atcactgata gggagtaaac tcgacatcgt tctctatcac
     6361 tgatagggag taaactcgac atacgttctc tatcactgat agggagtaaa ctcgaatgaa
     6421 gacgaaaggg cctcaggggc ccggtactaa ccaaactgga tctctgctgt ccctgtaata
     6481 aacccgaaaa ttttgaattt ttgtaatttg tttttgtaat tctttagttt gtatgtctgt
     6541 tgctattatg tctactattc tttcccctgc actgtacccc ccaatccccc cttttctttt
     6601 aaaattgtgg atgaatactg ccatttgtct gcagaattgg cgcacgcagt gccgatccgt
     6661 tcactaatcg aatggatctg tctctgtctc tctctccacc ttcttcttct attccttcgg
     6721 gcctgtcggg tcccctcggg gttgggaggt gggtctgaaa cgataatggt gaatatccct
     6781 gcctaactct attcactata gaaagtacag caaaaactat tcttaaacct accaagcctc
     6841 ctactatcat tatgaataat tttatatacc acagccaatt tgttatgtta aaccaattcc
     6901 acaaacttgc ccatttatct aattccaata attcttgttc attcttttct tgctggtttt
     6961 gcgattcttc aattaaggag tgtattaagc ttgtgtaatt gttaatttct ctgtcccact
     7021 ccatccaggt cgtgtgattc caaatctgtt ccagagattt attactccaa ctagcattcc
     7081 aaggcacagc agtggtgcaa atgagttttc cagagcaacc ccaaatcccc aggagctgtt
     7141 gatcctttag gtatctttcc acagccagga ttcttgcctg gagctgcttg atgccccaga
     7201 ctgtgagttg caacagatgc tgttgcgcct caatagccct cagcaaattg ttctgctgct
     7261 gcactatacc agacaataat tgtctggcct gtaccgtcag cgtcattgac gctgcgccca
     7321 tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc tcctattccc actgctcttt
     7381 tttctctctg caccactctt ctctttgcct tggtgggtgc tactcctaat ggttcaattt
     7441 ttactacttt atatttatat aattcacttc tccaattgtc cctcatatct cctcctccag
     7501 gtctgaagat cagcggccgg ccgcttgctg tgcggtggtc ttacttttgt tttgctcttc
     7561 ctctatcttg tctaaagctt ccttggtgtc ttttatctct atcctttgat gcacacaata
     7621 gagggttgct actgtattat ataatgatct aagttcttct gatcctgtct gaagggatgg
     7681 ttgtagctgt cccagtattt gtctacagcc ttctgatgtt tctaacaggc caggattaac
     7741 tgcgaatcgt tctagctccc tgcttgccca tactatatgt tttaatttat attttttctt
     7801 tccccctggc cttaaccgaa ttttttccca tcgcgatcta attctccccc gcttaatact
     7861 gacgctctcg cacccatctc tctccttcta gcctccgcta gtcaaaattt ttggcgtact
     7921 caccagtcgc cgcccctcgc ctcttgccgt gcgcgcttca gcaagccgag tcctgcgtcg
     7981 agagagctcc tctggtttcc ctttcgcttt caagtccctg ttcgggcgcc actgctagag
     8041 attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga
     8101 cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc
     8161 cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga cccagtacag
     8221 gcaaaacgcg ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa
     8281 tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact
     8341 cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg
     8401 cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat
     8461 gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca
     8521 tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc
     8581 caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg
     8641 cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca
     8701 gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac
     8761 taatgacccc gtaattgatt actattaata actaatgcat ggcggtaata cggttatcca
     8821 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagca
     8881 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     8941 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     9001 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     9061 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     9121 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     9181 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     9241 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     9301 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     9361 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9421 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9481 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9541 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9601 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9661 gtaacctgag gctatggcag ggcctgccgc cccgacgttg gctgcgagcc ctgggccttc
     9721 acccgaactt ggggggtggg gtggggaaaa ggaagaaacg cgggcgtatt ggccccaatg
     9781 gggtctcggt ggggtatcga cagagtgcca gccctgggac cgaaccccgc gtttatgaac
     9841 aaacgaccca acaccgtgcg ttttattctg tctttttatt gccgtcatag cgcgggttcc
     9901 ttccggtatt gtctccttcc gtgtttcagt tagcctcccc ctagggtggg cgaagaactc
     9961 cagcatgaga tccccgcgct ggaggatcat ccagccggcg tcccggaaaa cgattccgaa
    10021 gcccaacctt tcatagaagg cggcggtgga atcgaaatct cgtgatggca ggttgggcgt
    10081 cgcttggtcg gtcatttcga accccagagt cccgctcaga agaactcgtc aagaaggcga
    10141 tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca
    10201 gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag
    10261 cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc
    10321 atgatattcg gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg
    10381 ctcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga
    10441 tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc
    10501 gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca
    10561 gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc
    10621 acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg
    10681 caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcttg cagttcattc
    10741 agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg
    10801 aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc
    10861 tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat
    10921 cctcatcctg tctcttgatc gatctttgca aaagcctagg cctccaaaaa agcctcctca
    10981 ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat
    11041 tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg
    11101 agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca tacttctgcc
    11161 tgctggggag cctggggact ttccacacct ggttgctgac taattgagat gcatgctttg
    11221 catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac acacattcca
    11281 cagctggttc tttccgcctc aggactcttc ctttttcaat attattgaag catttatcag
    11341 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    11401 gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc
    11461 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
    11521 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
    11581 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
    11641 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
    11701 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
    11761 ctcaacccta tctcggtcta ttcttttgat ttatgctggc cttttgctca catgctaata
    11821 cgactcacta tagggcgaat tgggtacgtc cctaggcctc caaaaaagcc tcctcactac
    11881 ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt
    11941 cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt
    12001 aggggcggga tagctagagc cagacatgat aagatacatt gatgagtttg gacaaaccac
    12061 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
    12121 tgtaaccatt ataagctgca ataaacaagt tcctctcact ctctgatatt catttctttg
    12181 caagttataa atactgaata ataagatgac atgaactact actgctagag attttccaca
    12241 ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga cgggcacaca
    12301 ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc cctagttagc
    12361 cagagagctc ccaggctcag atctggtcta accagagaga cccagtacaa gcaaaaagca
    12421 gatcttgtct tcgttgggag tgaattagcc cttccagtcc ccccttttct tttaaaaagt
    12481 ggctaagatc tacagctgcc ttgtaagtca ttggtcttaa aggtacctca gaagaactcg
    12541 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
    12601 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
    12661 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
    12721 ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg
    12781 ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
    12841 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
    12901 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
    12961 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
    13021 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
    13081 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc
    13141 tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
    13201 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
    13261 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
    13321 atggatcctt atcatcgtgt ttttcaaagg aaaaccacgt ccccgtggtt cggggggcct
    13381 agacgttttt ttaacctcga ctaaacacat gtaaagcatg tgcaccgagg ccccagatca
    13441 gatcccatac aatggggtac cttctgggca tccttcagcc ccttgttgaa tacgcttgag
    13501 gagagccatt tgactctttc cacaactatc caactcacaa cgtggcactg gggttgtgcc
    13561 gcctttgcag gtgtatctta tacacgtggc ttttggccgc agaggcacct gtcgccaggt
    13621 ggggggttcc gctgcctgca aagggtcgct acagacgttg tttgtcttca agaagcttcc
    13681 agaggaactg cttccttcac gacattcaac agaccttgca ttcctttggc gagaggggaa
    13741 agacccctag gaatgctcgt caagaagaca gggccaggtt tccgggccct cacattgcca
    13801 aaagacggca atatggtgga aaataacata tagacaaacg cacaccggcc ttattccaag
    13861 cggcttcggc cagtaacgtt aggggggggg gagggagagg ggcggatgcg gccgcggatc
    13921 cataacttcg tataatgtat actatacgaa gttatttacc cggggagcat gtcaaggtca
    13981 aaatcgtcaa gggcatcggc tgggagcatg tctaagtcaa aatcgtcaag ggcgtcggtc
    14041 ggcccgccgc tttcgcactt tagctgtttc tccaggccac atatgattag ttccaggccg
    14101 aaaaggaagg caggttcggc tccctgccgg tcgaacagct caattgcttg tctcagaagt
    14161 gggggcatag aatcggtggt aggtgtctct ctttcctctt ttgctacttg atgctcctgt
    14221 tcctccaata cgcagcccag tgtaaagtgg cccacggcgg acagagcgta cagtgcgttc
    14281 tccagggaga agccttgctg acacaggaac gcgagctgat tttccagggt ttcgtactgt
    14341 ttctctgttg ggcgggtgcc gagatgcact ttagccccgt cgcgatgtga gaggagagca
    14401 cagcggtatg acttggcgtt gttccgcaga aagtcttgcc atgactcgcc ttccaggggg
    14461 cagaagtggg tatgatgcct gtccagcatc tcgattggca gggcatcgag cagggcccgc
    14521 ttgttcttca cgtgccagta cagggtaggc tgctcaactc ccagcttttg agcgagtttc
    14581 cttgtcgtca ggccttcgat accgacacca ttgagtaatt ccagagctcc gtttatgact
    14641 ttgctcttgt ccagcctaga catggtggca taacttcgta taatgtatac tatacgaagt
    14701 tatggatccg ctagcgtcta acaaaaaagc caaaaacggc cagaatttag cggacaattt
    14761 actagtctaa cactgaaaat tacatattga cccaaatgat tacatttcaa aaggtgccta
    14821 aaaaacttca caaaacacac tcgccaaccc cgagcgcata gttcaaaacc ggagcttcag
    14881 ctacttaaga agataggtac ataaaaccga ccaaagaaac tgac
//